HRGM_Genome_1166 - 11 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

c00007_HRGM_G.. (original name was: HRGM_Genome_1166_8) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	bacteriocin	101,901	117,732
Region 7.2	lanthipeptide	165,176	187,782

c00008_HRGM_G.. (original name was: HRGM_Genome_1166_9) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS-like	23,725	67,465	tundrenone	Other	11%

c00009_HRGM_G.. (original name was: HRGM_Genome_1166_10) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	bacteriocin	273,851	284,096

c00010_HRGM_G.. (original name was: HRGM_Genome_1166_11) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	NRPS,T1PKS	561,587	619,458	cystothiazole A	NRP + Polyketide	11%

c00011_HRGM_G.. (original name was: HRGM_Genome_1166_12) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	lanthipeptide	157,797	180,850
Region 11.2	NRPS	989,996	1,031,627
Region 11.3	NRPS,T3PKS	2,127,106	2,215,387	octapeptin C4	NRP	17%
Region 11.4	NRPS	2,317,123	2,426,497
Region 11.5	NRPS	2,749,682	2,797,575	bacillibactin	NRP	53%
Region 11.6	NRPS,betalactone	2,903,119	2,967,574

Region	Type	From	To	Most similar known cluster	Similarity
Region 7.1	bacteriocin	101,901	117,732
Region 7.2	lanthipeptide	165,176	187,782
Region 8.1	NRPS-like	23,725	67,465	tundrenone	Other	11%
Region 9.1	bacteriocin	273,851	284,096
Region 10.1	NRPS,T1PKS	561,587	619,458	cystothiazole A	NRP + Polyketide	11%
Region 11.1	lanthipeptide	157,797	180,850
Region 11.2	NRPS	989,996	1,031,627
Region 11.3	NRPS,T3PKS	2,127,106	2,215,387	octapeptin C4	NRP	17%
Region 11.4	NRPS	2,317,123	2,426,497
Region 11.5	NRPS	2,749,682	2,797,575	bacillibactin	NRP	53%
Region 11.6	NRPS,betalactone	2,903,119	2,967,574

No secondary metabolite regions were found in these records:: c00001_HRGM_G.. (original name was: HRGM_Genome_1166_1) (Genus species); c00002_HRGM_G.. (original name was: HRGM_Genome_1166_3) (Genus species); c00003_HRGM_G.. (original name was: HRGM_Genome_1166_4) (Genus species); c00004_HRGM_G.. (original name was: HRGM_Genome_1166_5) (Genus species); c00005_HRGM_G.. (original name was: HRGM_Genome_1166_6) (Genus species); c00006_HRGM_G.. (original name was: HRGM_Genome_1166_7) (Genus species)

Compact view

c00007_HRGM_G.. - Region 1 - bacteriocin

Download region GenBank file

Location: 101,901 - 117,732 nt. (total: 15,832 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

c00007_HRGM_G.. - Region 2 - lanthipeptide

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Location: 165,176 - 187,782 nt. (total: 22,607 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Lanthipeptides

ClusterBlast

KnownClusterBlast

SubClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for HRGM_Genome_1166_CDS_00359 - Putative Class III

HRGM_Genome_1166_CDS_00357 leader / core peptide: MNAVLELQKLEHDTDDKD - QAMIGLVDhaDhbVGPIKDhbIIDhbFDhbDhaDhbIKC
HRGM_Genome_1166_CDS_00358 leader / core peptide: MNAVLELQKLAHDTEGKDQAK - GFVDhaDhbVGPIDhbDhbIIDhbFDhbDhaDhbIDhaVKC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

Lanthipeptides

Core peptide predictions

HRGM_Genome_1166_CDS_00357 - Class III

Cleavage pHMM score: -0.00

RODEO score: 0

Monoisotopic mass: 2495.5 Da

Molecular weight: 2497.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2515.1 Da N = 1; 2533.1 Da N = 2; 2551.1 Da N = 3; 2569.1 Da N = 4; 2587.1 Da N = 5; 2605.2 Da N = 6

HRGM_Genome_1166_CDS_00358 - Class III

Cleavage pHMM score: 3.10

RODEO score: 0

Monoisotopic mass: 2209.3 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2; 2264.7 Da N = 3; 2282.7 Da N = 4; 2300.7 Da N = 5; 2318.7 Da N = 6; 2336.7 Da N = 7; 2354.8 Da N = 8

c00008_HRGM_G.. - Region 1 - NRPS-like

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Location: 23,725 - 67,465 nt. (total: 43,741 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

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Gene details

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NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 23724 - 67465:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_00433

Search NORINE for peptide: strict or relaxed

AMP-binding (244..657): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAygVGaVVK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 70% (weak)

c00009_HRGM_G.. - Region 1 - bacteriocin

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Location: 273,851 - 284,096 nt. (total: 10,246 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

c00010_HRGM_G.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 561,587 - 619,458 nt. (total: 57,872 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 561586 - 619458:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal) + (D-X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_01531

Search NORINE for peptide: strict or relaxed

AMP-binding (470..871): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAviVvAVwK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 60% (weak)

HRGM_Genome_1166_CDS_01532

PKS_AT (546..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.2
inactive: 128.6
Methoxymalonyl-CoA: 68.4
Methylmalonyl-CoA: 47.1
Propionyl-CoA: 34.6
Isobutyryl-CoA: 33.4
Ethylmalonyl-CoA: 22.0
CHC-CoA: 18.2
Benzoyl-CoA: 15.8
2-Methylbutyryl-CoA: 14.6
fatty_acid: 11.9
Acetyl-CoA: 5.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

HRGM_Genome_1166_CDS_01534

Search NORINE for peptide: strict or relaxed

AMP-binding (512..906): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALVgGmifK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 60% (weak)

HRGM_Genome_1166_CDS_01535

Search NORINE for peptide: strict or relaxed

AMP-binding (481..874): X

NRPSPredictor2: tyr, bht

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAsdTGEiVK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 70% (weak)

c00011_HRGM_G.. - Region 1 - lanthipeptide

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Location: 157,797 - 180,850 nt. (total: 23,054 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

Legend:

core biosynthetic genes

additional biosynthetic genes

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Lanthipeptides

ClusterBlast

KnownClusterBlast

SubClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for HRGM_Genome_1166_CDS_01773 - Putative Class II

HRGM_Genome_1166_CDS_01771 leader / core peptide: MTHEEIKLMNQSCGSGLKSLSMEEMQNIYGA - DhaDVDPRADhbPDhbIVADhaIIRDhaVAQDhaDhaKVCIDhaAVGDhaAIDhaGIVDhaNNKNCLG
HRGM_Genome_1166_CDS_01772 leader / core peptide: MNNSLEQSPVGKSLKSLPKEEMAAIFGG - DhbGMDAEVRDhaDhbLLCGMGIDhaFVADhaYLADhaAAFKCGKDNKN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

Lanthipeptides

Core peptide predictions

HRGM_Genome_1166_CDS_01771 - Class II

Cleavage pHMM score: 3.00

RODEO score: 0

Monoisotopic mass: 4386.4 Da

Molecular weight: 4389.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4407.0 Da N = 1; 4425.0 Da N = 2; 4443.0 Da N = 3; 4461.1 Da N = 4; 4479.1 Da N = 5; 4497.1 Da N = 6; 4515.1 Da N = 7; 4533.1 Da N = 8; 4551.2 Da N = 9

HRGM_Genome_1166_CDS_01772 - Class II

Cleavage pHMM score: 2.50

RODEO score: 0

Monoisotopic mass: 3746.8 Da

Molecular weight: 3749.3 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3767.3 Da N = 1; 3785.3 Da N = 2; 3803.4 Da N = 3; 3821.4 Da N = 4

c00011_HRGM_G.. - Region 2 - NRPS

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Location: 989,996 - 1,031,627 nt. (total: 41,632 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 989995 - 1031627:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_02483

Search NORINE for peptide: strict or relaxed

AMP-binding (468..863): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFFGiVYK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 90% (moderate)

c00011_HRGM_G.. - Region 3 - NRPS,T3PKS

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Location: 2,127,106 - 2,215,387 nt. (total: 88,282 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 2127105 - 2215387:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (X) + (asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 2127105 - 2168031:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 2161391 - 2202546:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 2164931 - 2215387:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_03504

Search NORINE for peptide: strict or relaxed

AMP-binding (568..968): asn

NRPSPredictor2: asn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DsTKIGhVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 80% (moderate)

HRGM_Genome_1166_CDS_03540

Search NORINE for peptide: strict or relaxed

AMP-binding (26..419): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGgTFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 90% (moderate)

HRGM_Genome_1166_CDS_03542

Search NORINE for peptide: strict or relaxed

AMP-binding (254..663): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAymVAAIiK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 70% (weak)

AMP-binding (1782..2190): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFwLGVTFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 90% (moderate)

c00011_HRGM_G.. - Region 4 - NRPS

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Location: 2,317,123 - 2,426,497 nt. (total: 109,375 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 7, location 2317122 - 2426497:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr - D-X) + (val - leu - tyr - D-leu) + (leu - val - D-X) + (leu - X - D-thr) + (val - val - D-X) + (orn - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_03665

Search NORINE for peptide: strict or relaxed

AMP-binding (469..871): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

AMP-binding (1518..1913): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVtnLAiiYK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 60% (weak)

AMP-binding (2559..2950): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVlqIGhVDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 70% (weak)

HRGM_Genome_1166_CDS_03666

Search NORINE for peptide: strict or relaxed

AMP-binding (473..869): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DALfmGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

AMP-binding (1521..1923): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAlFmGVTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

AMP-binding (2575..2970): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsNfAiiyK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

HRGM_Genome_1166_CDS_03667

Search NORINE for peptide: strict or relaxed

AMP-binding (470..858): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (1502..1897): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsNfAiiyK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

AMP-binding (2541..2940): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1166_CDS_03669

Search NORINE for peptide: strict or relaxed

AMP-binding (472..892): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (1556..1949): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (2611..3000): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVeqIGhVDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 70% (weak)

HRGM_Genome_1166_CDS_03670

Search NORINE for peptide: strict or relaxed

AMP-binding (472..865): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1533..1953): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (2622..3028): tyr

NRPSPredictor2: tyr

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGlITAEVvK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 80% (moderate)

AMP-binding (3674..4094): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1166_CDS_03671

Search NORINE for peptide: strict or relaxed

CAL_domain (256..564): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 29.4
AHBA: 14.8
Acetyl-CoA: 9.3
shikimic_acid: 0.0
NH2: 0.0

AMP-binding (1792..2191): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2865..3263): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvtNFaIVYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 70% (weak)

c00011_HRGM_G.. - Region 5 - NRPS

Download region GenBank file

Location: 2,749,682 - 2,797,575 nt. (total: 47,894 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 8, location 2749681 - 2797575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_04018

Search NORINE for peptide: strict or relaxed

AMP-binding (466..868): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQiGqIyK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 70% (weak)

AMP-binding (1556..1972): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1166_CDS_04020

Search NORINE for peptide: strict or relaxed

AMP-binding (31..526): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

c00011_HRGM_G.. - Region 6 - NRPS,betalactone

Download region GenBank file

Location: 2,903,119 - 2,967,574 nt. (total: 64,456 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 9, location 2903118 - 2967574:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile - D-X) + (D-ser) + (thr - D-phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1166_CDS_04162

Search NORINE for peptide: strict or relaxed

AMP-binding (468..866): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1896): phe

NRPSPredictor2: phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: phe, trp
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVgAVCK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 90% (moderate)

HRGM_Genome_1166_CDS_04163

Search NORINE for peptide: strict or relaxed

AMP-binding (261..671): dab

NRPSPredictor2: dab

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEISSIDK
Nearest Stachelhaus code:: dab
Stachelhaus code match:: 100% (strong)

AMP-binding (792..1011): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAqHtnLplK
Nearest Stachelhaus code:: hpg
Stachelhaus code match:: 50% (weak)

AMP-binding (1580..1982): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1166_CDS_04165

Search NORINE for peptide: strict or relaxed

AMP-binding (252..648): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

AMP-binding (1291..1674): X

NRPSPredictor2: phe, trp, phg, tyr, bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAciVAGVgK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)