HRGM_Genome_1161 - 4 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

c00008_HRGM_G.. (original name was: HRGM_Genome_1161_9) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS,T1PKS,transAT-PKS	85,942	162,895	xenocoumacin 1 / xenocoumacin II	NRP + Polyketide:Modular type I	28%
Region 8.2	betalactone	1,276,975	1,298,638	fengycin	NRP	40%
Region 8.3	betalactone	2,363,726	2,390,103
Region 8.4	terpene	3,505,123	3,527,009

No secondary metabolite regions were found in these records:: c00001_HRGM_G.. (original name was: HRGM_Genome_1161_1) (Genus species); c00002_HRGM_G.. (original name was: HRGM_Genome_1161_3) (Genus species); c00003_HRGM_G.. (original name was: HRGM_Genome_1161_4) (Genus species); c00004_HRGM_G.. (original name was: HRGM_Genome_1161_5) (Genus species); c00005_HRGM_G.. (original name was: HRGM_Genome_1161_6) (Genus species); c00006_HRGM_G.. (original name was: HRGM_Genome_1161_7) (Genus species); c00007_HRGM_G.. (original name was: HRGM_Genome_1161_8) (Genus species)

Compact view

c00008_HRGM_G.. - Region 1 - NRPS,T1PKS,transAT-PKS

Download region GenBank file

Location: 85,942 - 162,895 nt. (total: 76,954 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
transAT-PKS: (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 85941 - 162895:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - gly) + (ccmal) + (ccmal) + (mal - D-ile) + (val) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1161_CDS_00243

Search NORINE for peptide: strict or relaxed

PKS_AT (622..915): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 122.0
inactive: 64.4
Methoxymalonyl-CoA: 52.0
Methylmalonyl-CoA: 51.2
Ethylmalonyl-CoA: 30.2
Propionyl-CoA: 30.0
Isobutyryl-CoA: 20.5
Benzoyl-CoA: 19.7
3-Methylbutyryl-CoA: 14.2
Acetyl-CoA: 13.3
fatty_acid: 10.0
CHC-CoA: 9.0
2-Methylbutyryl-CoA: 8.5
trans-1,2-CPDA: 0.0

AMP-binding (2173..2560): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQiGVvWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 80% (moderate)

HRGM_Genome_1161_CDS_00244

PKS_AT (6..295): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 111.8
inactive: 39.2
Methylmalonyl-CoA: 38.2
Methoxymalonyl-CoA: 31.7
Propionyl-CoA: 20.6
CHC-CoA: 20.0
Isobutyryl-CoA: 19.8
2-Methylbutyryl-CoA: 13.0
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 10.7
Acetyl-CoA: 10.2
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

HRGM_Genome_1161_CDS_00247

Search NORINE for peptide: strict or relaxed

AMP-binding (1254..1651): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVYK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1161_CDS_00248

Search NORINE for peptide: strict or relaxed

AMP-binding (468..862): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWlGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

HRGM_Genome_1161_CDS_00249

PKS_AT (545..842): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 144.4
inactive: 90.4
Methoxymalonyl-CoA: 67.1
Methylmalonyl-CoA: 51.5
Propionyl-CoA: 40.1
Isobutyryl-CoA: 33.1
CHC-CoA: 25.8
Ethylmalonyl-CoA: 19.3
Acetyl-CoA: 18.4
Benzoyl-CoA: 15.0
2-Methylbutyryl-CoA: 13.0
3-Methylbutyryl-CoA: 9.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0

c00008_HRGM_G.. - Region 2 - betalactone

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Location: 1,276,975 - 1,298,638 nt. (total: 21,664 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

c00008_HRGM_G.. - Region 3 - betalactone

Download region GenBank file

Location: 2,363,726 - 2,390,103 nt. (total: 26,378 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

c00008_HRGM_G.. - Region 4 - terpene

Download region GenBank file

Location: 3,505,123 - 3,527,009 nt. (total: 21,887 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.