HRGM_Genome_1158 - 6 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

c00001_HRGM_G.. (original name was: HRGM_Genome_1158_1) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	bacteriocin	176,413	186,652
Region 1.2	lassopeptide	314,042	337,974	paeninodin	RiPP	40%
Region 1.3	NRPS	4,214,566	4,274,796

c00002_HRGM_G.. (original name was: HRGM_Genome_1158_2) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	183,358	204,203	carotenoid	Terpene	33%

c00003_HRGM_G.. (original name was: HRGM_Genome_1158_3) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS	148,338	193,792

c00008_HRGM_G.. (original name was: HRGM_Genome_1158_8) (Genus species)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	lassopeptide	151,006	174,895	paeninodin	RiPP	100%

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	bacteriocin	176,413	186,652
Region 1.2	lassopeptide	314,042	337,974	paeninodin	RiPP	40%
Region 1.3	NRPS	4,214,566	4,274,796
Region 2.1	terpene	183,358	204,203	carotenoid	Terpene	33%
Region 3.1	NRPS	148,338	193,792
Region 8.1	lassopeptide	151,006	174,895	paeninodin	RiPP	100%

No secondary metabolite regions were found in these records:: c00004_HRGM_G.. (original name was: HRGM_Genome_1158_4) (Genus species); c00005_HRGM_G.. (original name was: HRGM_Genome_1158_5) (Genus species); c00006_HRGM_G.. (original name was: HRGM_Genome_1158_6) (Genus species); c00007_HRGM_G.. (original name was: HRGM_Genome_1158_7) (Genus species); c00009_HRGM_G.. (original name was: HRGM_Genome_1158_9) (Genus species); c00010_HRGM_G.. (original name was: HRGM_Genome_1158_10) (Genus species); c00011_HRGM_G.. (original name was: HRGM_Genome_1158_11) (Genus species); c00012_HRGM_G.. (original name was: HRGM_Genome_1158_12) (Genus species); c00013_HRGM_G.. (original name was: HRGM_Genome_1158_13) (Genus species)

Compact view

c00001_HRGM_G.. - Region 1 - bacteriocin

Download region GenBank file

Location: 176,413 - 186,652 nt. (total: 10,240 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

c00001_HRGM_G.. - Region 2 - lassopeptide

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Location: 314,042 - 337,974 nt. (total: 23,933 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Lasso peptides

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Lasso peptides

Core peptide predictions

No core peptides found.

c00001_HRGM_G.. - Region 3 - NRPS

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Location: 4,214,566 - 4,274,796 nt. (total: 60,231 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

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NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 4214565 - 4274796:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val) + (ccmal) + (val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1158_CDS_04082

Search NORINE for peptide: strict or relaxed

AMP-binding (9..374): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAiWIGGvFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

HRGM_Genome_1158_CDS_04089

PKS_AT (6..275): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 47.5
Methoxymalonyl-CoA: 35.7
Methylmalonyl-CoA: 28.2
Propionyl-CoA: 26.2
inactive: 21.9
2-Methylbutyryl-CoA: 17.3
Ethylmalonyl-CoA: 10.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

HRGM_Genome_1158_CDS_04092

Search NORINE for peptide: strict or relaxed

AMP-binding (14..371): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DayFLGVVFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 80% (moderate)

c00002_HRGM_G.. - Region 1 - terpene

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Location: 183,358 - 204,203 nt. (total: 20,846 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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ClusterBlast

KnownClusterBlast

SubClusterBlast

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

c00003_HRGM_G.. - Region 1 - NRPS

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Location: 148,338 - 193,792 nt. (total: 45,455 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

ClusterBlast

KnownClusterBlast

SubClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Similar known gene clusters

No matches found.

Similar subclusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 148337 - 193792:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

HRGM_Genome_1158_CDS_04862

PKS_AT (6..289): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 70.2
Methylmalonyl-CoA: 41.9
Methoxymalonyl-CoA: 40.6
inactive: 30.9
Ethylmalonyl-CoA: 22.7
Propionyl-CoA: 10.9
Benzoyl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

HRGM_Genome_1158_CDS_04865

Search NORINE for peptide: strict or relaxed

AMP-binding (505..911): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

HRGM_Genome_1158_CDS_04866

Search NORINE for peptide: strict or relaxed

AMP-binding (8..426): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: iMlFLGGiiK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 60% (weak)

c00008_HRGM_G.. - Region 1 - lassopeptide

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Location: 151,006 - 174,895 nt. (total: 23,890 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

ClusterBlast

KnownClusterBlast

SubClusterBlast

Lasso peptides

Similar gene clusters

Similar known gene clusters

Similar subclusters

No matches found.

Lasso peptide predictions

Lassopeptide(s) for HRGM_Genome_1158_CDS_06105 - Putative Class II

HRGM_Genome_1158_CDS_06105 leader / core peptide / cleaved tail: MKKEYSKPALEVLDVKMTMA - GPGISIADSFQDDPDEIVHYS

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

HRGM_Genome_1158_CDS_06105 - Class II: Cleavage pHMM score: -12.40; RODEO score: 18; Monoisotopic mass: 2243.0 Da; Molecular weight: 2244.3 Da; Number of bridges: 0